• We derived ESC lines from blastocysts of the

  2018-11-02

  

  We derived ESC lines from blastocysts of the double transgenic mouse line Mlc2v-Cre; Rosa26-Yfp, in which Cre and Yfp are specifically knocked into the Mlc2v and Rosa26 gene loci, respectively. Mlc2v-Cre-mediated removal of a stop sequence in the Rosa26-YFP reporter line results in expression of YFP

• Inner pillar cells express Fgfr which may contribute to thei

  2018-11-02

  

  Inner pillar cells express Fgfr3, which may contribute to their capacity for regeneration (Jacques et al., 2007). Supporting cells in the chicken basilar papilla express Fgfr3, which is transiently downregulated during hair cell regeneration (Bermingham-McDonogh et al., 2001). Fgfr3−/− mice lack inn

• Differentiation of HD ESCs had only a minor

  2018-11-02

  

  Differentiation of HD ESCs had only a minor effect on repeat expansion; this was as expected considering the low level of somatic expansion found in most tissues. We did, however, find that differentiation status influenced the effect introduced oxidative DNA damage had on CAG expansion. Mouse model

• We can only speculate on the reasons for the contradictions

  2018-11-02

  

  We can only speculate on the reasons for the contradictions between our study and the Porrello study (Porrello et al., 2011). Importantly, we resected equal amounts of apex tissue, an obvious key parameter that otherwise could have influenced the outcome. Likewise, we used the same time schedule dur

• By continuously observing hundreds of individual HSCs and

  2018-11-02

  

  By continuously observing hundreds of individual HSCs and their progeny during differentiation, we can exclude a change in cell-cycle length in HSPCs upon GADD45G expression, which was unexpected, because studies on solid tissue na inhibitor reported a G2/M cell-cycle arrest mediated by GADD45G (Va

• A recent study indicated that XIST expression can be

  2018-11-02

  

  A recent study indicated that XIST expression can be lost upon prolonged passaging of female hiPSCs, referred to as “erosion of XCI” (Mekhoubad et al., 2012). This epigenetic erosion was found to be irreversible and correlated with a loss of differentiation characteristics and is very relevant for d

• br Experimental Procedures br Author Contributions br Acknow

  2018-11-02

  

  Experimental Procedures Author Contributions Acknowledgments The authors thank Sarah Chan and Jonathan Scolnick for technical assistance with deep sequencing and Yusuf Ali, Hiram Chipperfield, Rory Johnson, and Pauline Tay for their early work on the pancreatic differentiation protocol and

• br Experimental Procedures br Author

  2018-11-02

  

  Experimental Procedures Author Contributions Acknowledgments We are deeply thankful to V. Fossati and N. Lamas for helpful critical reading of the manuscript and to J. Goldman for generously providing the O4 antibody. We thank J.-Y. Li for providing access to wild-type mice for deriving mES

• br Experimental Procedures br Author Contributions br

  2018-11-02

  

  Experimental Procedures Author Contributions Acknowledgments We appreciate the expert technical assistance of N. Hayashi, K. Iseki, T. Hashiguchi, W. Ohashi, M. Kawahara, S. Okamoto, and C. Yamada (Laboratory for Retinal Regeneration, Center for Developmental Biology, RIKEN, Kobe). We appre

• There are a number of potential

  2018-11-02

  

  There are a number of potential challenges to using iPSC and iPSC-derived reductase enzyme to model human phenotype and disease. Somatic heterogeneity in iPSC lines that can occur during isolation and culture may interfere with examining genetic variants with subtle effects (Fusaki et al., 2009; In

• br Acknowledgments The study has been conducted with

  2018-11-02

  

  Acknowledgments The study has been conducted with the financial support of the Russian Foundation for Basic Research (Grant no. 15-01-07923). Introduction Studying the scattering processes of charged and neutral particles of various natures is one of the most important problems in 5ht recepto

• where and are the effective densities

  2018-11-02

  

  where and are the effective densities of states of electrons and holes in a two-dimensional subband, respectively; Let us assume that the equality is satisfied under optical excitation, and find the dependence of the position of the quasi Fermi levels of electrons and holes on the temperature and

• As a result of correctly matching this

  2018-11-02

  

  As a result of correctly matching this area of the radio sky to the optical sky, ten radio sources with the flux density of more than 6.8mJy were identified with the stars brighter than 10 and one object was identified with a diffuse image (ESO-538-10, no. 10 in Table 1). Eight radio objects (stars

• br Results Table presents basic descriptive information of o

  2018-11-02

  

  Results Table 1 presents basic descriptive information of our data. The estimates are presented first for all tracts, then for rural and urban tracts separately, with F-test p-values for testing rural-urban differences. Average marginal effects from multivariate fractional logit regression estim

• In this context companies face the

  2018-11-02

  

  In this context, companies face the process of building and maintaining relationships that prove to be valuable for both customers and the company. Considering a proposal of integrating the TOE model (Technology–Organization–Environment) and the focus on relationship management applied to the m-mark

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